Research Article

Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry

  1. Fumio Nomura1,*,
  2. Takeshi Tomonaga1,
  3. Kazuyuki Sogawa1,
  4. Tatsuya Ohashi1,
  5. Masahiko Nezu1,
  6. Masahiko Sunaga1,
  7. Naoki Kondo2,
  8. Masaomi Iyo3,
  9. Hideaki Shimada4,
  10. Takenori Ochiai4

Article first published online: 3 FEB 2004

DOI: 10.1002/pmic.200300674

Issue

PROTEOMICS

PROTEOMICS

Volume 4, Issue 4, pages 1187–1194, April 2004

Additional Information

How to Cite

Nomura, F., Tomonaga, T., Sogawa, K., Ohashi, T., Nezu, M., Sunaga, M., Kondo, N., Iyo, M., Shimada, H. and Ochiai, T. (2004), Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry. PROTEOMICS, 4: 1187–1194. doi: 10.1002/pmic.200300674

Author Information

  1. 1

    Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan

  2. 2

    Seimei Hospital, Fuji City, Shizuoka, Japan

  3. 3

    Department of Psychiatry, Graduate School of Medicine, Chiba University, Chiba, Japan

  4. 4

    Department of Surgery 2, Graduate School of Medicine, Chiba University, Chiba, Japan

*Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba City, Chiba, Japan 260-8670 Fax: +81-43-226-2324

Publication History

  1. Issue published online: 23 MAR 2004
  2. Article first published online: 3 FEB 2004
  3. Manuscript Received: 27 JUN 2003

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Keywords:

  • Alcoholism;
  • γ-Glutamyltransferase;
  • Surface-enhanced laser desorption/ionization

Abstract

Since personal and verbal reporting of alcohol use is not necessarily accurate, objective markers to assess alcohol consumption are required. The currently available markers, however, are limited in sensitivity and specificity for screening of excessive alcohol drinkers. Therefore, searches for novel markers are warranted. Recently, surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) has been successfully used to detect disease-associated proteins in complex biological specimens. We used the ProteinChip SELDI technology to generate comparative protein profiles of the consecutive serum samples obtained during abstinence from a total of 16 chronic alcoholic patients hospitalized for a rehabilitation program. We recognized two peaks (5.9 and 7.8 kDa), both of which had been downregulated on admission, the expression level of which significantly increased after a one-week abstinence. These changes were also seen in nonresponders of γ-glutamyltransferase. These two proteins were partially purified and subjected to amino acid sequencing. The 5.9 kDa protein was identified as a fragment of fibrinogen αE chain and the 7.8 kDa was a fragment of apoprotein A-II. These novel protein fragments may be promising biomarkers for excessive alcohol drinking.

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