Research Article

Proteomic profiling of proteins decreased in hepatocellular carcinoma from patients infected with hepatitis C virus

  1. Yuichiro Yokoyama1,2,
  2. Yasuhiro Kuramitsu2,
  3. Motonari Takashima3,
  4. Norio Iizuka4,
  5. Toshifusa Toda5,
  6. Shuji Terai1,
  7. Isao Sakaida1,
  8. Masaaki Oka3,
  9. Kazuyuki Nakamura2,*,
  10. Kiwamu Okita1

Article first published online: 22 APR 2004

DOI: 10.1002/pmic.200300712

Issue

PROTEOMICS

PROTEOMICS

Special Issue: Proceedings of the AEPS Meeting, Melbourne, Australia 27-28 September 2003

Volume 4, Issue 7, pages 2111–2116, July 2004

Additional Information

How to Cite

Yokoyama, Y., Kuramitsu, Y., Takashima, M., Iizuka, N., Toda, T., Terai, S., Sakaida, I., Oka, M., Nakamura, K. and Okita, K. (2004), Proteomic profiling of proteins decreased in hepatocellular carcinoma from patients infected with hepatitis C virus. PROTEOMICS, 4: 2111–2116. doi: 10.1002/pmic.200300712

Author Information

  1. 1

    Department of Gastroenterology and Hepatology

  2. 2

    Department of Biochemistory and Biomolecular Recognition

  3. 3

    Department of Surgery II

  4. 4

    Department of Bioregulatory Function, Yamaguchi University School of Medicine, Yamaguchi, Japan

  5. 5

    Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan

*Department of Biochemistory and Biomolecular recognition, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi, 755-8505, Japan Fax: +81-836-22-2212

Publication History

  1. Issue published online: 22 JUN 2004
  2. Article first published online: 22 APR 2004
  3. Manuscript Received: 24 SEP 2003

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (116K)

Keywords:

  • Hepatitis C virus;
  • Hepatocellular carcinoma;
  • Liver proteome;
  • Two-dimensional gel electrophoresis

Abstract

Hepatocellular carcinoma (HCC) is a major cause of death in Japan. It has been suggested that hepatitis C virus (HCV) plays an important role in hepatocarcinogenesis, because of high incidence among the patients. To understand the mechanism of hepatocarcinogenesis after HCV infection, we performed a comparative study on the protein profiles between tumorous and nontumorous specimens from the patients infected with HCV by means of two-dimensional electrophoresis. Eleven spots were decreased in HCC tissues from over 50% of the patients. Eight proteins out of 11 spots were identified using peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. These proteins were liver type aldolase, tropomyosin β-chain, ketohexokinase, enoyl-CoA hydratase, albumin, smoothelin, ferritin light chain, and arginase 1. The intensity of enoyl-CoA hydratase, tropomyosin β-chain, ketohexokinase, liver type aldolase, and arginase 1 was significantly different (p < 0.05). The decrease of 8 proteins was characteristic in HCC. We will discuss the implication of these proteins for the loss of function of hepatocytes and for the possibility of carcinogenesis of HCV-related HCC.

Get PDF (116K)