Short Communication

Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients

  1. Debora Dumont,
  2. Jean-Paul Noben,
  3. Jef Raus,
  4. Piet Stinissen,
  5. Johan Robben*

Article first published online: 22 APR 2004

DOI: 10.1002/pmic.200300715

Issue

PROTEOMICS

PROTEOMICS

Special Issue: Proceedings of the AEPS Meeting, Melbourne, Australia 27-28 September 2003

Volume 4, Issue 7, pages 2117–2124, July 2004

Additional Information

How to Cite

Dumont, D., Noben, J.-P., Raus, J., Stinissen, P. and Robben, J. (2004), Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients. PROTEOMICS, 4: 2117–2124. doi: 10.1002/pmic.200300715

Author Information

  1. Biomedisch Onderzoeksinstituut (BIOMED), Limburgs Universitair Centrum and School of Life Sciences, Transnational University Limburg, Belgium

*Biomedisch Onderzoeksinstituut (BIOMED), Universitaire Campus gebouw A, 3590 Diepenbeek, Belgium Fax: +32-11-26-92-09

Publication History

  1. Issue published online: 22 JUN 2004
  2. Article first published online: 22 APR 2004
  3. Manuscript Received: 6 OCT 2003

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Keywords:

  • Cerebrospinal fluid;
  • Liquid chromatography;
  • Mass spectrometry;
  • Multiple sclerosis;
  • Two-dimensional gel electrophoresis

Abstract

Multiple sclerosis is an autoimmune inflammatory demyelinating disease of the central nervous system. Disease mechanisms in multiple sclerosis at the molecular level remain poorly understood and no reliable proteinaceous disease markers are available yet. The goal of the present study is the construction of a protein database of two-dimensional gel electrophoresis (2-DE) separated cerebrospinal fluid (CSF) proteins from multiple sclerosis patients. By means of liquid chromatography tandem mass spectrometry 65 different proteins were identified from 300 spots. Eighteen of these proteins have not been reported previously on 2-DE gels of CSF. Here we report on the identification of these proteins and discuss their potential relation to multiple sclerosis.

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