Cardiovascular-related proteins identified in human plasma by the HUPO Plasma Proteome Project Pilot Phase

Authors

  • Beniam T. Berhane,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    2. Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA
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  • Chenggong Zong,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • David A. Liem,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • Aaron Huang,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • Steven Le,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • Ricky D. Edmondson,

    1. National Center for Toxicological Research, Jefferson, AR, USA
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  • Richard C. Jones,

    1. National Center for Toxicological Research, Jefferson, AR, USA
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  • Xin Qiao,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • Julian P. Whitelegge,

    1. Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA
    2. Department of Psychiatry and Biobehavioral Sciences, UCLA, Los Angeles, CA, USA
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  • Peipei Ping,

    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
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  • Thomas M. Vondriska

    Corresponding author
    1. Departments of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    • Cardiovascular Research Laboratories, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine at UCLA, Room 1619, MRL Building, Los Angeles, CA 90095, USA Fax: +1-310-267-5623
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Abstract

Proteomic profiling of accessible bodily fluids, such as plasma, has the potential to accelerate biomarker/biosignature development for human diseases. The HUPO Plasma Proteome Project pilot phase examined human plasma with distinct proteomic approaches across multiple laboratories worldwide. Through this effort, we confidently identified 3020 proteins, each requiring a minimum of two high-scoring MS/MS spectra. A critical step subsequent to protein identification is functional annotation, in particular with regard to organ systems and disease. Performing exhaustive literature searches, we have manually annotated a subset of these 3020 proteins that have cardiovascular-related functions on the basis of an existing body of published information. These cardiovascular-related proteins can be organized into eight groups: markers of inflammation and/or cardiovascular disease, vascular and coagulation, signaling, growth and differentiation, cytoskeletal, transcription factors, channels/receptors and heart failure and remodeling. In addition, analysis of the peptide per protein ratio for MS/MS identification reveals group-specific trends. These findings serve as a resource to interrogate the functions of plasma proteins, and moreover, the list of cardiovascular-related proteins in plasma constitutes a baseline proteomic blueprint for the future development of biosignatures for diseases such as myocardial ischemia and atherosclerosis.

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