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Proteomic characterization of the cytotoxic mechanism of gold (III) porphyrin 1a, a potential anticancer drug

Authors

  • Ying Wang,

    1. Department of Chemistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
    2. Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
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  • Qing-Yu He,

    1. Department of Chemistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
    2. Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
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  • Chi-Ming Che Professor,

    1. Department of Chemistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
    2. Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
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    • Additional corresponding author

  • Jen-Fu Chiu Professor

    Corresponding author
    1. Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
    2. Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China
    • Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong SAR, P. R. China Fax: +852-2817-1006
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Abstract

There has been increasing interest in the potential applications of gold (III) complexes as anticancer drugs with higher cytotoxicity and fewer side effects than existing metal anticancer drugs. Our previous findings demonstrated that gold (III) porphyrin 1a preferentially induced apoptosis in a cancer cell line (SUNE1). In this study, we identified differentially expressed proteins related to the drug's cytotoxic action by comparing the protein alterations induced by gold (III) porphyrin 1a and cisplatin treatments. Several clusters of altered proteins were identified, including cellular structure and stress-related chaperone proteins, proteins involved in reactive oxygen species and enzyme proteins, translation factors, proteins that mediate cell proliferation or differentiation, and proteins participating in the internal degradation systems. Our results indicated that multiple factors leading to apoptosis were involved in drug cytotoxicity in SUNE1 cells. The balance between pro-apoptotic and anti-apoptotic signals determined the final fate of cancer cells.

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