Rapid protein identification using direct infusion nanoelectrospray ionization mass spectrometry

Authors

  • Sixue Chen Dr.

    Corresponding author
    1. Proteomics and Mass Spectrometry Facility, Donald Danforth Plant Science Center, St. Louis, USA
    • Proteomics and Mass Spectrometry Facility, Donald Danforth Plant Science Center, 975 N. Warson Road, St. Louis, MO 63146, USA Fax: +1-314-587-1324
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Abstract

Current protein identification techniques are largely based on MALDI-TOF mass fingerprinting and LC-ESI MS/MS sequence tag analysis. Here we describe an improved method for rapid protein identification that uses direct infusion nanoelectrospray quadrupole time-of-flight (nanoESI QTOF) MS. Protein digests were analyzed without LC separation using nanoESI on a QSTAR XL MS/MS system in information dependent data acquisition mode. The protein identification conditions and parameters were extensively evaluated with in-solution and in-gel digested protein samples. Rapid identification of proteins was achieved and compared directly to the results obtained on the same samples using nanoflow HPLC-MS/MS on the QSTAR system. The increased throughput, reproducibility, the high data quality, and the ease of use make the direct infusion system an efficient and affordable technique for protein identification analysis.

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