Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish

Authors

  • Marianne M. Zupanc,

    1. School of Engineering and Science, International University Bremen, Bremen, Germany
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  • Ursula M. Wellbrock,

    1. School of Engineering and Science, International University Bremen, Bremen, Germany
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  • Günther K. H. Zupanc Prof. Dr.

    Corresponding author
    1. School of Engineering and Science, International University Bremen, Bremen, Germany
    • International University Bremen, School of Engineering and Science, P.O. Box 750 561, 28725 Bremen, Germany Fax: +49-421-200-493244
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    • Dedicated to Theodore H. Bullock, Professor Emeritus at the University of California, San Diego, on the occasion of his 90th birthday on 16 May 2005.


Abstract

In contrast to mammals, adult teleost fish exhibit an enormous potential to regenerate neuronal tissue after injuries to the CNS. By combining a well-defined cerebellar lesion paradigm with differential proteome analysis at a post-lesion survival time of 3 days, we screened for protein candidates involved in repair of the fish brain. Out of nearly 900 protein spots detected on 2-D gels, spot intensity was significantly increased at least twofold in 30 spots and decreased to at least half the intensity of control tissue in 23 spots. The proteins associated with 24 of the spots were identified by PMF and MS/MS fragmentation. The cellular localization and the spatiotemporal patterns of two of these proteins, beta-actin and beta-tubulin, were further characterized through immunohistochemistry. Comparison of the observed changes in protein abundance with the previously characterized events underlying regeneration of the cerebellum suggests that the proteins identified are especially involved in cellular proliferation and survival, as well as axonal sprouting.

Ancillary