Is protein overlap in two-dimensional gels a serious practical problem?
Article first published online: 23 JAN 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 6, Issue 5, pages 1374–1375, No. 5 March 2006
How to Cite
Hunsucker, S. W. and Duncan, M. W. (2006), Is protein overlap in two-dimensional gels a serious practical problem?. Proteomics, 6: 1374–1375. doi: 10.1002/pmic.200500521
- Issue published online: 2 MAR 2006
- Article first published online: 23 JAN 2006
- Manuscript Received: 12 JUL 2005
- Protein expression analysis;
- Protein quantification;
- Proteome dynamic range
In a recently published article, Campostrini et al. [Proteomics 2005, 5, 2385–2395] raised questions regarding the utility of 2-D gels in proteomics research. We believe that the authors have overlooked several key issues including the dynamic range of protein expression and the sensitivity of the analytical methods used to explore a proteome. We argue that 2-D gels have and will continue to provide meaningful quantitative data when applied to proteomic analysis and that the practical significance of spot overlap has been overstated.