Protein stains for proteomic applications: Which, when, why?

Authors

  • Ingrid Miller,

    Corresponding author
    1. Institut für Medizinische Chemie, Department für Naturwissenschaften, Veterinärmedizinische Universität Wien, Wien, Austria
    • Institut für Medizinische Chemie, Department für Naturwissenschaften, Veterinärmedizinische Universität Wien, Veterinärplatz 1, 1210 Wien, Austria Fax: +43-1-25077-4290
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  • Johanne Crawford,

    1. Nonlinear Dynamics Ltd, Newcastle-upon-Tyne, UK
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  • Elisabetta Gianazza

    1. Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche, Università degli Studi di Milano, Milano, Italy
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Abstract

This review recollects literature data on sensitivity and dynamic range for the most commonly used colorimetric and fluorescent dyes for general protein staining, and summarizes procedures for the most common PTM-specific detection methods. It also compiles some important points to be considered in imaging and evaluation. In addition to theoretical considerations, examples are provided to illustrate differential staining of specific proteins with different detection methods. This includes a large body of original data on the comparative evaluation of several pre- and post-electrophoresis stains used in parallel on a single specimen, horse serum run in 2-DE (IPG-DALT). A number of proteins/protein spots are found to be over- or under-revealed with some of the staining procedures.

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