Both these authors contributed equally to this work.
MascotDatfile: An open-source library to fully parse and analyse MASCOT MS/MS search results
Article first published online: 4 JAN 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 7, Issue 3, pages 364–366, No. 3 February 2007
How to Cite
Helsens, K., Martens, L., Vandekerckhove, J. and Gevaert, K. (2007), MascotDatfile: An open-source library to fully parse and analyse MASCOT MS/MS search results. Proteomics, 7: 364–366. doi: 10.1002/pmic.200600682
- Issue published online: 2 FEB 2007
- Article first published online: 4 JAN 2007
- Manuscript Received: 8 SEP 2006
- the Fund for Scientific Research – Flanders, Belgium (F.W.O Vlaanderen)
- Protein identification
MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT results into an easily accessible and fully functional object model (http://genesis.ugent.be/MascotDatfile). Several scripts based on this library are provided as examples, allowing direct automation of important routine tasks involved in processing peptide identifications.