Proteomic characterization of copper stress response in Cannabis sativa roots

Authors

  • Elisa Bona,

    1. Department of Environmental and Life Sciences, University of Piemonte Orientale “A. Avogadro”, Alessandria, Italy
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  • Francesco Marsano,

    1. Department of Environmental and Life Sciences, University of Piemonte Orientale “A. Avogadro”, Alessandria, Italy
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  • Maria Cavaletto Dr.,

    Corresponding author
    1. Department of Environmental and Life Sciences, University of Piemonte Orientale “A. Avogadro”, Alessandria, Italy
    • Department of Environmental and Life Sciences, University of Piemonte Orientale “A. Avogadro”, Via Bellini 25/G, 15100 Alessandria, Italy Fax: +390131360390
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    • These authors contributed equally to the work.

  • Graziella Berta

    1. Department of Environmental and Life Sciences, University of Piemonte Orientale “A. Avogadro”, Alessandria, Italy
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    • These authors contributed equally to the work.


Abstract

Cannabis sativa is an annual herb with very high biomass and capability to absorb and accumulate heavy metals in roots and shoots; it is therefore a good candidate for phytoremediation of soils contaminated with metals. Copper is an essential micronutrient for all living organisms, it participates as an important redox component in cellular electron transport chains; but is extremely toxic to plants at high concentrations. The aim of this work was to investigate copper effects on the root proteome of C. sativa, whose genome is still unsequenced. Copper stress induced the suppression of two proteins, the down-regulation of seven proteins, while five proteins were up-regulated. The resulting differences in protein expression pattern were indicative of a plant adaptation to chronic stress and were directed to the reestablishment of the cellular and redox homeostasis.

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