Plant Proteomics

Effects of agricultural production systems and their components on protein profiles of potato tubers

  1. Satu J. Lehesranta1,6,
  2. Kaisa M. Koistinen1,7,
  3. Nathalie Massat2,
  4. Howard V. Davies3,
  5. Louise V. T. Shepherd3,
  6. James W. McNicol2,
  7. Ismail Cakmak4,
  8. Julia Cooper5,
  9. Lorna Lück5,
  10. Sirpa O. Kärenlampi1,
  11. Carlo Leifert Professor5,*

Article first published online: 19 FEB 2007

DOI: 10.1002/pmic.200600889

Issue

PROTEOMICS

PROTEOMICS

Volume 7, Issue 4, pages 597–604, No. 4 February 2007

Additional Information

How to Cite

Lehesranta, S. J., Koistinen, K. M., Massat, N., Davies, H. V., Shepherd, L. V. T., McNicol, J. W., Cakmak, I., Cooper, J., Lück, L., Kärenlampi, S. O. and Leifert, C. (2007), Effects of agricultural production systems and their components on protein profiles of potato tubers. PROTEOMICS, 7: 597–604. doi: 10.1002/pmic.200600889

Author Information

  1. 1

    Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland

  2. 2

    Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland

  3. 3

    Quality, Health and Nutrition Programme, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland

  4. 4

    Faculty of Engineering and Natural Science, Sabanci University, Istanbul, Turkey

  5. 5

    Nafferton Ecological Farming Group, Newcastle University, Nafferton Farm, Stocksfield, UK

  6. 6

    Current address: Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland

  7. 7

    Current address: School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK

*Nafferton Ecological Farming Group, Newcastle University, Nafferton Farm, Stocksfield NE43 7XD, UK Fax: +44-1661-831006

Publication History

  1. Issue published online: 19 FEB 2007
  2. Article first published online: 19 FEB 2007
  3. Manuscript Received: 9 NOV 2006

Funded by

  • SAFE FOODS (EU FP6 Contract No. Food-CT-2004-506446)
  • QualityLowInputFood (EU FP6 Contract No. CT-2004-506358)
  • Scottish Executive Environment and Rural Affairs Department (S. E. E. R. A. D.)

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Keywords:

  • 2-DE;
  • Conventional cultivation;
  • Organic cultivation;
  • Solanum tuberosum L.

Abstract

A range of studies have compared the level of nutritionally relevant compounds in crops from organic and nonorganic farming systems, but there is very limited information on the effect of farming systems and their key components on the protein composition of plants. We addressed this gap by quantifying the effects of different farming systems and key components of such systems on the protein profiles of potato tubers. Tuber samples were produced in the Nafferton factorial systems study, a group of long-term, replicated factorial field experiments designed to identify and quantify the effect of fertility management methods, crop protection practices and rotational designs used in organic, low input and conventional production systems. Protein profiles were determined by 2-DE and subsequent protein identification by HPLC-ESI-MS/MS. Principal component analysis of 2-DE data showed that only fertility management practices (organic matter vs. mineral fertiliser based) had a significant effect on protein composition. Quantitative differences were detected in 160 of the 1100 tuber proteins separated by 2-DE. Proteins identified by MS are involved in protein synthesis and turnover, carbon and energy metabolism and defence responses, suggesting that organic fertilisation leads to an increased stress response in potato tubers.

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