Protein Arrays

Immunodominant Francisella tularensis antigens identified using proteome microarray. ©Crown Copyright 2007 Dstl

  1. Jim E. Eyles Dr.1,*,
  2. Berkay Unal2,
  3. Margaret Gill Hartley1,
  4. Sarah L. Newstead1,
  5. Helen Flick-Smith1,
  6. Joann L. Prior1,
  7. Petra C. F. Oyston1,
  8. Arlo Randall3,4,
  9. Yunxiang Mu2,
  10. Siddiqua Hirst2,
  11. Douglas M. Molina5,
  12. D. Huw Davies2,
  13. Tim Milne1,
  14. Kate F. Griffin1,
  15. Pierre Baldi3,4,
  16. Rick W. Titball1,6,
  17. Philip L. Felgner2

Article first published online: 29 MAY 2007

DOI: 10.1002/pmic.200600985

Issue

PROTEOMICS

PROTEOMICS

Volume 7, Issue 13, pages 2172–2183, No. 13 June 2007

Additional Information

How to Cite

Eyles, J. E., Unal, B., Hartley, M. G., Newstead, S. L., Flick-Smith, H., Prior, J. L., Oyston, P. C. F., Randall, A., Mu, Y., Hirst, S., Molina, D. M., Davies, D. H., Milne, T., Griffin, K. F., Baldi, P., Titball, R. W. and Felgner, P. L. (2007), Immunodominant Francisella tularensis antigens identified using proteome microarray. ©Crown Copyright 2007 Dstl. PROTEOMICS, 7: 2172–2183. doi: 10.1002/pmic.200600985

Author Information

  1. 1

    Defence Science and Technology Laboratory, Porton Down, Salisbury, UK

  2. 2

    University of California, Department of Medicine, Irvine, CA, USA

  3. 3

    School of Information and Computer Sciences, University of California, Irvine, CA, USA

  4. 4

    Institute for Genomics and Bioinformatics, University of California, Irvine, CA, USA

  5. 5

    ImmPORT Therapeutics, One Technology, Irvine, CA, USA

  6. 6

    Department of Infectious and Tropical Disease, London School of Hygiene and Tropical Medicine, London, UK

*Building 7A, DSTL, Porton Down, SP4 0JQ, UK Fax: +44-1980-614-307

Publication History

  1. Issue published online: 26 JUN 2007
  2. Article first published online: 29 MAY 2007
  3. Manuscript Received: 8 DEC 2006

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Keywords:

  • Adjuvant;
  • Microarray;
  • Tularemia;
  • Vaccine

Abstract

Stimulation of protective immune responses against intracellular pathogens is difficult to achieve using non-replicating vaccines. BALB/c mice immunized by intramuscular injection with killed Francisella tularensis (live vaccine strain) adjuvanted with preformed immune stimulating complexes admixed with CpG, were protected when systemically challenged with a highly virulent strain of F. tularensis (Schu S4). Serum from immunized mice was used to probe a whole proteome microarray in order to identify immunodominant antigens. Eleven out of the top 12 immunodominant antigens have been previously described as immunoreactive in F. tularensis. However, 31 previously unreported immunoreactive antigens were revealed using this approach. Twenty four (50%) of the ORFs on the immunodominant hit list belonged to the category of surface or membrane associated proteins compared to only 22% of the entire proteome. There were eight hypothetical protein hits and eight hits from proteins associated with different aspects of metabolism. The chip also allowed us to readily determine the IgG subclass bias, towards individual or multiple antigens, in protected and unprotected animals. These data give insight into the protective immune response and have potentially important implications for the rational design of non-living vaccines for tularemia and other intracellular pathogens.

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