Animal Proteomics

Antigen discovery: A postgenomic approach to paratuberculosis diagnosis

  1. Baptiste Leroy1,
  2. Virginie Roupie2,
  3. Isabelle Noël-Georis1,3,
  4. Valérie Rosseels2,
  5. Karl Walravens4,
  6. Marc Govaerts4,
  7. Kris Huygen2,
  8. Ruddy Wattiez Professor1,*

Article first published online: 15 MAR 2007

DOI: 10.1002/pmic.200600988

Issue

PROTEOMICS

PROTEOMICS

Volume 7, Issue 7, pages 1164–1176, No. 7 April 2007

Additional Information

How to Cite

Leroy, B., Roupie, V., Noël-Georis, I., Rosseels, V., Walravens, K., Govaerts, M., Huygen, K. and Wattiez, R. (2007), Antigen discovery: A postgenomic approach to paratuberculosis diagnosis. PROTEOMICS, 7: 1164–1176. doi: 10.1002/pmic.200600988

Author Information

  1. 1

    Department of Proteomics and Protein Biochemistry, University of Mons-Hainaut, Mons, Belgium

  2. 2

    Laboratory of Mycobacterial Immunology, WIV-Pasteur Institute, Brussels, Belgium

  3. 3

    Institut de Recherches J.-M. Wiame (IRMW), Bruxelles, Belgium

  4. 4

    Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Uccle, Belgium

*Department of Proteomics and Protein Biochemistry. University of Mons-Hainaut, Av. du Champs de Mars, 6, B-7000 Mons, Belgium Fax: +32-65373320

Publication History

  1. Issue published online: 27 MAR 2007
  2. Article first published online: 15 MAR 2007
  3. Manuscript Received: 8 DEC 2006

Funded by

  • Wallon Government (Belgium) – Recherche Initiative5
  • Research Associate of the National Funds for Scientific Research

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Keywords:

  • Diagnostic antigens;
  • ELISA;
  • Immunoproteomics;
  • Mycobacterium avium subsp. paratuberculosis

Abstract

Paratuberculosis is a chronic enteritis caused in domestic and wild ruminant species by Mycobacterium avium subsp. paratuberculosis (MAP) that is responsible for major economic losses to the agricultural industry. To date, no satisfactory therapeutic, vaccine, or diagnostic tools are available, globally impairing all control programs. In this study, we have undertaken a large-scale postgenomic analysis of MAP proteins, to identify specific antigens that could potentially improve the diagnosis of paratuberculosis. Two complementary approaches were implemented, the first one consisting in the systematic proteomic identification of proteins present in MAP culture filtrates (CFs), followed by the selection of MAP-specific proteins by BLAST query on available mycobacterial genomes. The resulting database represents the first established secretome of MAP and a useful source of potentially specific antigens. The second approach consisted in the immunoproteomic analysis of both MAP extracts and CFs, using sera from MAP-infected and uninfected cattle. Combining results obtained with both approaches resulted in the identification of 25 candidate diagnostic antigens. Five of these were tested in an ELISA assay for their diagnostic potential, on a limited panel of field sera, and the combination of three of them competed in performance with available commercial assays, reaching a test sensitivity of 94.74% and specificity of 97.92%.

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