Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels

Authors


Abstract

In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons:

  1. Low price

  2. Visible with the eye

  3. Desk top scanners can be employed for image acquisition

  4. Better for quantitative analysis than silver staining

  5. Possible modifications for fast or highly sensitive staining

  6. Mass spectrometry compatible

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