This paper is dedicated to the memory of Hans Kende (1937-2006), esteemed colleague and friend.
Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum†
Article first published online: 6 AUG 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 7, Issue 17, pages 3171–3183, No. 17 September 2007
How to Cite
Paper, J. M., Scott-Craig, J. S., Adhikari, N. D., Cuomo, C. A. and Walton, J. D. (2007), Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum. Proteomics, 7: 3171–3183. doi: 10.1002/pmic.200700184
- Issue published online: 5 SEP 2007
- Article first published online: 6 AUG 2007
- Manuscript Received: 19 FEB 2007
- Fusarium head blight;
High-throughput MS/MS was used to identify proteins secreted by Fusarium graminearum (Gibberella zeae) during growth on 13 media in vitro and in planta during infection of wheat heads. In vitro secreted proteins were collected from the culture filtrates, and in planta proteins were collected by vacuum infiltration. A total of 289 proteins (229 in vitro and 120 in planta) were identified with high statistical confidence. Forty-nine of the in planta proteins were not found in any of the in vitro conditions. The majority (91–100%) of the in vitro proteins had predicted signal peptides, but only 56% of the in planta proteins. At least 13 of the nonsecreted proteins found only in planta were single-copy housekeeping enzymes, including enolase, triose phosphate isomerase, phosphoglucomutase, calmodulin, aconitase, and malate dehydrogenase. The presence of these proteins in the in planta but not in vitro secretome might indicate that significant fungal lysis occurs during pathogenesis. On the other hand, several of the proteins lacking signal peptides that were found in planta have been reported to be potent immunogens secreted by animal pathogenic fungi, and therefore could be important in the interaction between F. graminearum and its host plants.