Both these authors contributed equally to this work.
Membrane subproteomic analysis of Mycobacterium bovis bacillus Calmette-Guérin
Article first published online: 5 OCT 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 7, Issue 21, pages 3919–3931, No. 21 November 2007
How to Cite
Zheng, J., Wei, C., Leng, W., Dong, J., Li, R., Li, W., Wang, J., Zhang, Z. and Jin, Q. (2007), Membrane subproteomic analysis of Mycobacterium bovis bacillus Calmette-Guérin. Proteomics, 7: 3919–3931. doi: 10.1002/pmic.200700342
- Issue published online: 25 OCT 2007
- Article first published online: 5 OCT 2007
- Manuscript Received: 3 APR 2007
- Integral membrane protein;
- Membrane subproteome;
- Mycobacterium bovis bacillus Calmette-Guérin;
- Transmembrane helix
Mycobacterium bovis bacillus Calmette–Guérin (BCG) vaccine has been known for a long time to prevent tuberculosis (TB) worldwide since 1921. Nonetheless, we know little about BCG membrane proteome. In the present study, we utilized alkaline incubation and Triton X-114-based methods to enrich BCG membrane proteins and subsequently digested them using proteolytic enzyme. The recovered peptides were further separated by 2-D LC and identified by ESI-MS/MS. As a result, total 474 proteins were identified, including 78 integral membrane proteins (IMPs). Notably, 18 BCG IMPs were described for the first time in mycobacterium. Further analysis of the 78 IMPs indicated that the theoretical molecular mass distribution of them ranged from 8.06 to 167.86 kDa and pI scores ranged from 4.40 to 11.60. Functional classification revealed that a large proportion of the identified IMPs (67.9%, 53 out of 78) were involved in cell wall and cell processes functional group. In conclusion, here we reported a comprehensive profile of the BCG membrane subproteome. The present investigation may allow the identification of some valuable vaccine and drug target candidates and thus provide basement for future designing of preventive, diagnostic, and therapeutic strategies against TB.