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Automated technologies and novel techniques to accelerate protein crystallography for structural genomics

Authors

  • Babu A. Manjasetty Dr.,

    Corresponding author
    1. Case Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, USA
    2. Case Center for Proteomics and Mass Spectrometry, Case Western Reserve University, Cleveland, OH, USA
    • Center for Synchrotron Biosciences, Case Center for Proteomics, National Synchrotron Light Source, Upton NY 11973, USA Fax: +1-631-344-5594
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  • Andrew P. Turnbull,

    1. Cancer Research Technology, Birkbeck College, University of London, London, UK
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  • Santosh Panjikar,

    1. EMBL Hamburg c/o DESY, Hamburg, Germany
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  • Konrad Büssow,

    1. Protein Structure Factory, Helmholtz Center for Infection Research, Division of Structural Biology, Braunschweig, Germany
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  • Mark R. Chance Professor

    1. Case Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, USA
    2. Case Center for Proteomics and Mass Spectrometry, Case Western Reserve University, Cleveland, OH, USA
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    • Additional corresponding author


Abstract

The sequence infrastructure that has arisen through large-scale genomic projects dedicated to protein analysis, has provided a wealth of information and brought together scientists and institutions from all over the world. As a consequence, the development of novel technologies and methodologies in proteomics research is helping to unravel the biochemical and physiological mechanisms of complex multivariate diseases at both a functional and molecular level. In the late sixties, when X-ray crystallography had just been established, the idea of determining protein structure on an almost universal basis was akin to an impossible dream or a miracle. Yet only forty years after, automated protein structure determination platforms have been established. The widespread use of robotics in protein crystallography has had a huge impact at every stage of the pipeline from protein cloning, over-expression, purification, crystallization, data collection, structure solution, refinement, validation and data management- all of which have become more or less automated with minimal human intervention necessary. Here, recent advances in protein crystal structure analysis in the context of structural genomics will be discussed. In addition, this review aims to give an overview of recent developments in high throughput instrumentation, and technologies and strategies to accelerate protein structure/function analysis.

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