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Technology
DigesTip: A new device for a rapid and efficient in-solution protein digestion
Article first published online: 2 MAY 2008
DOI: 10.1002/pmic.200700873
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Marangoni, R., Chiarini, R., Iannone, G. and Salerno, M. (2008), DigesTip: A new device for a rapid and efficient in-solution protein digestion. PROTEOMICS, 8: 2165–2167. doi: 10.1002/pmic.200700873
Publication History
- Issue published online: 4 JUN 2008
- Article first published online: 2 MAY 2008
- Manuscript Received: 24 SEP 2007
- Abstract
- References
- Cited By
Keywords:
- Fragment coverage;
- Immobilized trypsin;
- Mass spectrometry;
- Protein digestion
Abstract
DigesTip is a new device for in-solution protein digestion, based on a patent pending technology, able to immobilize enzymes (trypsin, in this case) on a solid surface, keeping their activity preserved. DigesTip is a standard pipette tip, usable both by human and by robots. Its main performances are: very short digestion time (1 min) and usability with low protein sample concentrations (5 μg/mL). DigesTip obtains a clear signal in MS measurements and its usage rules out several preparative steps.