Dissecting the proteome of pea mature seeds reveals the phenotypic plasticity of seed protein composition

Authors

  • Michael Bourgeois,

    Corresponding author
    1. Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines (UMR-LEG), Institut National de la Recherche Agronomique (INRA), Bretenières, France
    • UMR-LEG, INRA, Domaine d'Epoisses, 21110 Bretenières, France Fax: +33-3-80-69-32-63
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  • Françoise Jacquin,

    1. Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines (UMR-LEG), Institut National de la Recherche Agronomique (INRA), Bretenières, France
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  • Vincent Savois,

    1. Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines (UMR-LEG), Institut National de la Recherche Agronomique (INRA), Bretenières, France
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  • Nicolas Sommerer,

    1. Unité de Recherche Protéomique, INRA, Montpellier, France
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  • Valérie Labas,

    1. Unité Mixte de Recherche Physiologie de la Reproduction et des Comportements, INRA/Centre National de la Recherche Scientifique (CNRS)/Haras Nationaux/Université de Tours, Nouzilly, France
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  • Céline Henry,

    1. Unité de Biochimie Bactérienne, INRA, Domaine de Vilvert, France
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  • Judith Burstin

    1. Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines (UMR-LEG), Institut National de la Recherche Agronomique (INRA), Bretenières, France
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Abstract

Pea (Pisum sativum L.) is the most cultivated European pulse crop and the pea seeds mainly serve as a protein source for monogastric animals. Because the seed protein composition impacts on seed nutritional value, we aimed at identifying the determinants of its variability. This paper presents the first pea mature seed proteome reference map, which includes 156 identified proteins (http://www.inra.fr/legumbase/peaseedmap/). This map provides a fine dissection of the pea seed storage protein composition revealing a large diversity of storage proteins resulting both from gene diversity and post-translational processing. It gives new insights into the pea storage protein processing (especially 7S globulins) as a possible adaptation towards progressive mobilization of the proteins during germination. The nonstorage seed proteome revealed the presence of proteins involved in seed defense together with proteins preparing germination. The plasticity of the seed proteome was revealed for seeds produced in three successive years of cultivation, and 30% of the spots were affected by environmental variations. This work pinpoints seed proteins most affected by environment, highlighting new targets to stabilize storage protein composition that should be further analyzed.

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