Research Article

Identification of novel Stat6 regulated proteins in IL-4-treated mouse lymphocytes

  1. Soile Tuomela1,2,†,
  2. Kirsi J. Rautajoki1,2,†,
  3. Robert Moulder1,
  4. Tuula A. Nyman1,3,
  5. Riitta Lahesmaa Professor1,*

Article first published online: 29 JAN 2009

DOI: 10.1002/pmic.200800161

Issue

PROTEOMICS

PROTEOMICS

Special Issue: PROTEOMICS Reviews 2009

Volume 9, Issue 4, pages 1087–1098, No. 4 February 2009

Additional Information

How to Cite

Tuomela, S., Rautajoki, K. J., Moulder, R., Nyman, T. A. and Lahesmaa, R. (2009), Identification of novel Stat6 regulated proteins in IL-4-treated mouse lymphocytes. PROTEOMICS, 9: 1087–1098. doi: 10.1002/pmic.200800161

Author Information

  1. 1

    Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland

  2. 2

    Turku Graduate School of Biomedical Sciences, University of Turku, Turku, Finland

  3. 3

    Institute of Biotechnology, University of Helsinki, Helsinki, Finland

  1. †

    These authors have contributed equally to this work.

*Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistökatu 6A, 5th floor, 20521 Turku, Finland Fax: +358-2-333-8000

Publication History

  1. Issue published online: 11 FEB 2009
  2. Article first published online: 29 JAN 2009
  3. Manuscript Received: 18 FEB 2008

Funded by

  • National Technology Agency of Finland (Tekes)
  • Academy of Finland
  • Turku University Hospital Research Fund
  • Jenny and Antti Wihuri Foundation
  • Finnish Concordia Fund
  • Sigrid Juselius Foundation

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Keywords:

  • 2-DE;
  • CBFb2;
  • CNBP;
  • Mass spectrometry;
  • Stat6

Abstract

Interleukin 4 (IL-4) has an indispensable role in the differentiation of naive T helper (Th) cells toward the Th2 phenotype and induction of B cells to produce the IgE class of Igs. By regulating these two cell types, IL-4 has a pre-eminent role in regulation of allergic inflammation. IL-4-mediated regulation of T and B cell functions is largely transmitted through signal transducer and activator of transcription 6 (Stat6). In this study, we have used metabolic labeling and 2-D electrophoresis to detect differences in the proteomes of IL-4 stimulated spleen mononuclear cells of Stat6−/− and wild type mice and MS/MS for protein identification. With this methodology, we identified 49 unique proteins from 21 protein spots to be differentially expressed. Interestingly, in Stat6−/− CD4+ cells the expression of isoform 2 of core binding factor b (CBFb2) was enhanced. CBFb is a non-DNA binding cofactor for the Runx family of transcription factors, which have been implicated in regulation of Th cell differentiation. We also found cellular nucleic acid protein (CNBP) to be downregulated in Stat6−/− cells. None of the proteins identified in this study have previously been reported to be regulated via Stat6. The results highlight the importance of exploiting proteomics tools to complement the studies on Stat6 target genes identified through transcriptional profiling.

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