Bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions

Authors

  • Roberto A. Bobadilla Fazzini Dr.,

    Corresponding author
    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
    2. Departamento de Prevención de Riesgos y Medio Ambiente, Facultad de Ciencias de la Construcción y Ordenamiento Territorial, Universidad Tecnológica Metropolitana, Santiago de Chile, Chile
    • Departamento de Prevención de Riesgos y Medio Ambiente, Universidad Tecnológica Metropolitana, Calle Dieciocho No. 390, Santiago de Chile, Chile Fax: +56-2-787-7321
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  • Agata Bielecka,

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
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  • Ana K. Poucas Quintas,

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
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  • Peter N. Golyshin,

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
    2. Current address: School of Biological Sciences, Bangor University, LH57 2DG Bangor, Wales, UK.
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  • Maria J. Preto,

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
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  • Kenneth N. Timmis,

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
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  • Vitor A. P. Martins dos Santos

    1. Environmental Microbiology Lab, HZI-Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
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Abstract

In this study, the stable consortium composed by Pseudomonas reinekei strain MT1 and Achromobacter xylosoxidans strain MT3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. The analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. Carbon-limiting studies showed a higher metabolic versatility in the community through upregulation of parallel catabolic enzymes (salicylate 5-hydroxylase and 17-fold on 2-keto-4-pentenoate hydratase) indicating a possible alternative carbon routing in the upper degradation pathway highlighting the effect of minor proportions of strain MT3 over the major consortia component strain MT1 with a significant change in the expression levels of the enzymes of the mainly induced biodegradation pathway such as salicylate 1-hydroxylase and catechol 1,2-dioxygenase together with important changes in the outer membrane composition of P. reinekei MT1 under different culture conditions. The study has demonstrated the importance of the outer membrane as a sensing/response protective barrier caused by interspecies interactions highlighting the role of the major outer membrane proteins OprF and porin D in P. reinekei sp. MT1 under the culture conditions tested.

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