A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions

Authors

  • Andreas O. Helbig,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
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  • Marco J. L. de Groot,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
    Current affiliation:
    1. Information and Communication Theory Group, Delft University of Technology, Mekelweg 4, Delft, 2628 CD, The Netherlands
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  • Renske A. van Gestel,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
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  • Shabaz Mohammed,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
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  • Erik A. F. de Hulster,

    1. Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    2. Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands
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  • Marijke A. H. Luttik,

    1. Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    2. Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands
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  • Pascale Daran-Lapujade,

    1. Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    2. Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands
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  • Jack T. Pronk,

    1. Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    2. Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands
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  • Albert J. R. Heck,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
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  • Monique Slijper

    Corresponding author
    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    2. Netherlands Proteomics Centre, Utrecht, The Netherlands
    • Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands Fax: +31-302518219
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Abstract

To investigate the effect of anaerobiosis on the Saccharomyces cerevisiae mitochondrial proteome and the formation of respiratory chain and other protein complexes, we analyzed mitochondrial protein extracts that were enriched from lysates of aerobic and anaerobic steady-state chemostat cultures. We chose an innovative approach in which native mitochondrial membrane protein complexes were separated by 1-D blue native PAGE, which was combined with quantitative analysis of each complex subunit using stable isotope labeling. LC-FT(ICR)-MS/MS analysis was applied to identify and quantify the mitochondrial proteins. In addition, to establish if changes in mitochondrial complex composition occurred under anaerobiosis, we investigated the 1-D blue native PAGE protein migration patterns by Pearson correlation analysis. Surprisingly, we discovered that under anaerobic conditions, where the yeast respiratory chain is not active, the respiratory chain supercomplexes, such as complex V dimer, complex (III)2(IV)2 and complex (III)2(IV) were still present, although at reduced levels. Pearson correlation analysis showed that the composition of the mitochondrial complexes was unchanged under aerobic or anaerobic conditions, with the exception of complex II. In addition, this latter approach allowed screening for possible novel complex interaction partners, since for example protein Aim38p, with a yet unknown function, was identified as a possible component of respiratory chain complex IV.

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