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Copper stress proteomics highlights local adaptation of two strains of the model brown alga Ectocarpus siliculosus

Authors

  • Andrés Ritter,

    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    3. Departamento de Ecología, Center for Advanced Studies in Ecology & Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile
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  • Martin Ubertini,

    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Sarah Romac,

    1. Computer and Genomics resource Centre, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Fanny Gaillard,

    1. Computer and Genomics resource Centre, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Ludovic Delage,

    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Aaron Mann,

    1. Departamento de Ecología, Center for Advanced Studies in Ecology & Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile
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  • J. Mark Cock,

    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Thierry Tonon,

    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
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  • Juan A. Correa,

    1. Departamento de Ecología, Center for Advanced Studies in Ecology & Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile
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  • Philippe Potin

    Corresponding author
    1. Université Pierre et Marie Curie-Paris 6, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    2. CNRS, Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, Roscoff, France
    • UPMC-Paris 6, CNRS, UMR 7139 Végétaux Marins et Biomolécules, Station Biologique, Place Georges Teissier, BP74, F 29682, Roscoff, France Fax: +33-2-98292324
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  • Publication of this paper was delayed after final acceptance due to awaiting publication of the Ectocarpus genome paper by Cock et al. 1 and the public release of the genome data.

Abstract

Ectocarpus siliculosus is a cosmopolitan brown alga with capacity to thrive in copper enriched environments. Analysis of copper toxicity was conducted in two strains of E. siliculosus isolated from (i) an uncontaminated coast in southern Peru (Es32) and (ii) a copper polluted rocky beach in northern Chile (Es524). Es32 was more sensitive than Es524, with toxicity detected at 50 μg/L Cu, whereas Es524 displayed negative effects only when exposed to 250 μg/L Cu. Differential soluble proteome profiling for each strain exposed to sub-lethal copper levels allowed to identify the induction of proteins related to processes such as energy production, glutathione metabolism as well as accumulation of HSPs. In addition, the inter-strain comparison of stress-related proteomes led to identify features related to copper tolerance in Es524, such as striking expression of a PSII Mn-stabilizing protein and a Fucoxanthine chlorophyll a–c binding protein. Es524 also expressed specific stress-related enzymes such as RNA helicases from the DEAD box families and a vanadium-dependent bromoperoxidase. These observations were supported by RT-qPCR for some of the identified genes and an enzyme activity assay for vanadium-dependent bromoperoxidase. Therefore, the occurrence of two different phenotypes within two distinct E. siliculosus strains studied at the physiological and proteomic levels strongly suggest that persistent copper stress may represent a selective force leading to the development of strains genetically adapted to copper contaminated sites.

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