Global analysis of the rat and human platelet proteome – the molecular blueprint for illustrating multi-functional platelets and cross-species function evolution

Authors

  • Yanbao Yu,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
    2. Department of Biochemistry and Biophysics, UNC-Duke Michael Hooker Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
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    • These authors contributed equally to this work.

  • Taohua Leng,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
    2. Department of Biochemistry and Biophysics, UNC-Duke Michael Hooker Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
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    • These authors contributed equally to this work.

  • Dong Yun,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
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    • These authors contributed equally to this work.

  • Na Liu,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
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  • Jun Yao,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
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  • Ying Dai,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
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  • Pengyuan Yang,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
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  • Xian Chen

    Corresponding author
    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China
    2. Department of Biochemistry and Biophysics, UNC-Duke Michael Hooker Proteomics Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    • Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA Fax: +1-919-966-2852
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Abstract

Emerging evidences indicate that blood platelets function in multiple biological processes including immune response, bone metastasis and liver regeneration in addition to their known roles in hemostasis and thrombosis. Global elucidation of platelet proteome will provide the molecular base of these platelet functions. Here, we set up a high-throughput platform for maximum exploration of the rat/human platelet proteome using integrated proteomic technologies, and then applied to identify the largest number of the proteins expressed in both rat and human platelets. After stringent statistical filtration, a total of 837 unique proteins matched with at least two unique peptides were precisely identified, making it the first comprehensive protein database so far for rat platelets. Meanwhile, quantitative analyses of the thrombin-stimulated platelets offered great insights into the biological functions of platelet proteins and therefore confirmed our global profiling data. A comparative proteomic analysis between rat and human platelets was also conducted, which revealed not only a significant similarity, but also an across-species evolutionary link that the orthologous proteins representing “core proteome”, and the “evolutionary proteome” is actually a relatively static proteome.

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