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MassSieve: Panning MS/MS peptide data for proteins

Authors

  • Douglas J. Slotta,

    Corresponding author
    1. Laboratory of Neurotoxicology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
    • NCBI/NLM/NIH, 45 Center Drive, Rm 5AS21, Bethesda, MD 20892, USA Fax: +1-301-48-2484
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  • Melinda A. McFarland,

    1. Laboratory of Neurotoxicology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
    Current affiliation:
    1. Center for Food Safety and Applied Nutrition, Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD 20740, USA
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  • Sanford P. Markey

    1. Laboratory of Neurotoxicology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
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Abstract

We present MassSieve, a Java-based platform for visualization and parsimony analysis of single and comparative LC-MS/MS database search engine results. The success of mass spectrometric peptide sequence assignment algorithms has led to the need for a tool to merge and evaluate the increasing data set sizes that result from LC-MS/MS-based shotgun proteomic experiments. MassSieve supports reports from multiple search engines with differing search characteristics, which can increase peptide sequence coverage and/or identify conflicting or ambiguous spectral assignments.

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