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A new generation of cross-linkers for selective detection by MALDI MS

Authors

  • David Paramelle,

    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
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  • Sonia Cantel,

    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
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  • Christine Enjalbal,

    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
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  • Muriel Amblard,

    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
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  • Eric Forest,

    1. Proteins Mass Spectrometry Laboratory, Institut de Biologie Structurale, CEA, CNRS, UJF, UMR 5075, Grenoble, France
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  • Michaël Heymann,

    1. Institut de Biologie et Chimie des Protéines, Laboratoire de Bioinformatique et RMN structurales, Pole BioInformatique Lyonnais, UMR 5086 CNRS – Université Lyon 1, Lyon, France
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  • Christophe Geourjon,

    1. Institut de Biologie et Chimie des Protéines, Laboratoire de Bioinformatique et RMN structurales, Pole BioInformatique Lyonnais, UMR 5086 CNRS – Université Lyon 1, Lyon, France
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  • Jean Martinez,

    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
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    • These authors contributed equally to this work.

  • Gilles Subra

    Corresponding author
    1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Universités Montpellier 1 et 2, Montpellier, France
    • Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier, France Fax: +33-4-67-54-86-54
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    • These authors contributed equally to this work.


Abstract

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged cross-linker JMV 3378 in conjunction with a neutral MALDI matrix α-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the α-cyano-4-hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome c and apo-myoglobine as model proteins.

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