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Use of selected reaction monitoring data for label-free quantification of protein modification stoichiometry

Authors

  • Deepa Balasubramaniam,

    1. Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
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  • Christie L. Eissler,

    1. Department of Biochemistry, Purdue University, West Lafayette, IN, USA
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  • Cynthia V. Stauffacher,

    1. Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
    2. Center for Cancer Research, Purdue University, West Lafayette, IN, USA
    3. Markey Center for Structural Biology, Purdue University, West Lafayette, IN, USA
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  • Mark C. Hall

    Corresponding author
    1. Department of Biochemistry, Purdue University, West Lafayette, IN, USA
    2. Center for Cancer Research, Purdue University, West Lafayette, IN, USA
    • Department of Biochemistry, Purdue University, 175 S. University St., West Lafayette, IN 47907, USA Fax: +1-765-494-7897
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Abstract

Quantification of protein and PTM abundance in biological samples is an important component of proteomic studies. Label-free methods for quantification using MS are attractive because they are simple to implement and applicable to any experimental system. We demonstrate that PTM stoichiometry can be accurately measured using label-free quantification and selected reaction monitoring. Use of selected reaction monitoring is advantageous with complex biological samples and we show this approach can be used to quantify multiple PTMs independently on a single peptide.

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