Protein abundances are more conserved than mRNA abundances across diverse taxa

Authors

  • Jon M. Laurent,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
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    • These authors contributed equally to this work.

  • Christine Vogel,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
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    • These authors contributed equally to this work.

  • Taejoon Kwon,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
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  • Stephanie A. Craig,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
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  • Daniel R. Boutz,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
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  • Holly K. Huse,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
    2. Section of Molecular Genetics and Microbiology, University of Texas at Austin, TX, USA
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  • Kazunari Nozue,

    1. Department of Plant Pathology, University of California, Davis, CA, USA
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  • Harkamal Walia,

    1. Department of Plant Pathology, University of California, Davis, CA, USA
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  • Marvin Whiteley,

    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
    2. Section of Molecular Genetics and Microbiology, University of Texas at Austin, TX, USA
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  • Pamela C. Ronald,

    1. Department of Plant Pathology, University of California, Davis, CA, USA
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  • Edward M. Marcotte

    Corresponding author
    1. Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX, USA
    2. Department of Chemistry and Biochemistry, University of Texas at Austin, TX, USA
    • Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX 78712, USA Fax: +1-512-232-3432
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Abstract

Proteins play major roles in most biological processes; as a consequence, protein expression levels are highly regulated. While extensive post-transcriptional, translational and protein degradation control clearly influence protein concentration and functionality, it is often thought that protein abundances are primarily determined by the abundances of the corresponding mRNAs. Hence surprisingly, a recent study showed that abundances of orthologous nematode and fly proteins correlate better than their corresponding mRNA abundances. We tested if this phenomenon is general by collecting and testing matching large-scale protein and mRNA expression data sets from seven different species: two bacteria, yeast, nematode, fly, human, and rice. We find that steady-state abundances of proteins show significantly higher correlation across these diverse phylogenetic taxa than the abundances of their corresponding mRNAs (p=0.0008, paired Wilcoxon). These data support the presence of strong selective pressure to maintain protein abundances during evolution, even when mRNA abundances diverge.

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