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Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach

Authors

  • Dongli He,

    1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China
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  • Chao Han,

    1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China
    2. Graduate University of Chinese Academy of Sciences, Beijing, P. R. China
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  • Jialing Yao,

    1. College of life science, Huazhong Agriculture University, Wuhan, P. R. China
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  • Shihua Shen,

    1. The Research and Development Center for Energy Plants, Institute of Botany, Chinese Academy of Sciences, Beijing, P. R. China
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  • Pingfang Yang

    Corresponding author
    1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China
    • Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, P. R. China Fax: +86-27-87510956
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  • Colour Online: See article online to view figures 1, 2, 4, 5 in colour.

Abstract

Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.

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