Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana

Authors

  • Zhe Jenny Zhang,

    1. Department of Biochemistry, Interdisciplinary Plant Group, and Bond Life Sciences Center, University of Missouri, Columbia, MO, USA
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  • Scott C. Peck

    Corresponding author
    1. Department of Biochemistry, Interdisciplinary Plant Group, and Bond Life Sciences Center, University of Missouri, Columbia, MO, USA
    • 271H Life Science Center, 1201 Rollins St, University of Missouri, Columbia, MO 65211, USA Fax: +1-573-884-9395
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  • Colour Online: See the article online to view Figs. 1 and 6 in colour.

Abstract

The plasma membrane (PM) serves as the point of contact between cells and the outside environment. As such, changes in the PM proteome are an important component of understanding cellular responses to a diverse array of stimuli. However, intricate sample handling to enrich PM proteomes by traditional methods is both technically challenging and time consuming. Here, we describe a simplified method for decreasing the representation of other membrane-containing organelles such as the endoplasmic reticulum, plastids and mitochondria from crude microsomal membrane isolations. The decrease in other organellar proteomes results in an increase in both the total number of PM proteins and the number of spectra identified from these proteins representing the PM proteome. Therefore, this strategy represents a simple and rapid method for enriching PM proteins from Arabidopsis cell cultures for proteomic analyses.

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