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Sites of ubiquitin attachment in Saccharomyces cerevisiae

Authors

  • Lea M. Starita,

    1. Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA
    2. Department of Genome Sciences, University of Washington, Seattle, WA, USA
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    • These authors contributed equally in this work.

  • Russell S. Lo,

    1. Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA
    2. Department of Genome Sciences, University of Washington, Seattle, WA, USA
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    • These authors contributed equally in this work.

  • Jimmy K. Eng,

    1. Department of Genome Sciences, University of Washington, Seattle, WA, USA
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  • Priska D. von Haller,

    1. Department of Genome Sciences, University of Washington, Seattle, WA, USA
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  • Stanley Fields

    Corresponding author
    1. Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA
    2. Department of Genome Sciences, University of Washington, Seattle, WA, USA
    3. Department of Medicine, University of Washington, Seattle, WA, USA
    • Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA Fax: +1-206-543-0754
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  • Colour Online: See the article online to view Fig. 2 in colour.

Abstract

Sites of ubiquitin modification have been identified by mass spectrometry based on the increase in molecular mass of a tryptic peptide carrying two additional glycine residues from the ubiquitin moiety. However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae.

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