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Proteomic analysis of the effects of baicalein on colorectal cancer cells

Authors

  • Wen-Shih Huang,

    1. Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan
    2. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan
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  • Yi-Hung Kuo,

    1. Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan
    2. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan
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  • Chih-Chien Chin,

    1. Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan
    2. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan
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  • Jeng-Yi Wang,

    1. Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan
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  • Hong-Ren Yu,

    1. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan
    2. Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan
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  • Jiunn-Ming Sheen,

    1. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan
    2. Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan
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  • Shui-Yi Tung,

    1. Department of Hepato-Gastroenterological, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan
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  • Chien-Heng Shen,

    1. Department of Hepato-Gastroenterological, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan
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  • Te-Chuan Chen,

    1. Division of Nephrology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan
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  • Mei-Lan Sung,

    1. Department of Nursing, Chang Gung University of Science and Technology, Taiwan
    2. Chronic Diseases and Health Promotion Research Center, CGUST, Taiwan
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  • Hwey-Fang Liang,

    1. Department of Nursing, Chang Gung University of Science and Technology, Taiwan
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  • Hsing-Chun Kuo

    Corresponding author
    1. Department of Nursing, Chang Gung University of Science and Technology, Taiwan
    2. Chronic Diseases and Health Promotion Research Center, CGUST, Taiwan
    • Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan
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Correspondence: Dr. Hsing-Chun Kuo,

Department of Nursing, Chang Gung University of Science and Technology, Chia-Yi Campus, No.2, Chia-Pu Rd. West Sec. Putz, Chia-yi 61363, Taiwan

E-mail: guscsi@gmail.com

Fax: +886-5-3628866

Abstract

Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS-PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein-treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein-treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up-regulating the levels of peroxiredoxin-6 (PRDX6). Knockdown of PRDX6 in baicalein-treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up-regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.

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