Inter-organ proteomic analysis reveals insights into the molecular mechanisms underlying the anti-diabetic effects of cis-9, trans-11-conjugated linoleic acid in ob/ob mice

Authors

  • Vanessa Rungapamestry,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    2. Nutrigenomics Research Group, UCD Conway Institute & UCD Institute of Food and Health, School of Public Health & Population Science, University College Dublin, Dublin, Ireland
    Search for more papers by this author
  • Jolene McMonagle,

    1. Nutrigenomics Research Group, UCD Conway Institute & UCD Institute of Food and Health, School of Public Health & Population Science, University College Dublin, Dublin, Ireland
    Search for more papers by this author
  • Clare Reynolds,

    1. Nutrigenomics Research Group, UCD Conway Institute & UCD Institute of Food and Health, School of Public Health & Population Science, University College Dublin, Dublin, Ireland
    Search for more papers by this author
  • Garry Rucklidge,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Martin Reid,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Gary Duncan,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Karen Ross,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Graham Horgan,

    1. Biomathematics and Statistics Scotland, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Sinead Toomey,

    1. Nutrigenomics Research Group, UCD Conway Institute & UCD Institute of Food and Health, School of Public Health & Population Science, University College Dublin, Dublin, Ireland
    Search for more papers by this author
  • Aidan P. Moloney,

    1. Teagasc, Animal and Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland
    Search for more papers by this author
  • Baukje de Roos,

    1. University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK
    Search for more papers by this author
  • Helen M. Roche

    Corresponding author
    1. Nutrigenomics Research Group, UCD Conway Institute & UCD Institute of Food and Health, School of Public Health & Population Science, University College Dublin, Dublin, Ireland
    • Nutrigenomics Research Group, UCD Conway Institute, UCD School of Public Health, University College Dublin, Belfield, Dublin 4, Ireland Fax: +353-1-7167101
    Search for more papers by this author

  • Colour Online: See the article online to view Fig. 1 in colour.

Abstract

cis-9, trans-11-Conjugated linoleic acid (c9 t11 CLA) exerts anti-diabetic effects by improving systemic insulin sensitivity and inflammation. Levels of CLA in beef can be increased by feeding cattle on pasture. This study aimed to explore the efficacy of a CLA-rich diet (0.6% w/w c9 t11 CLA), presented as beef enriched with CLA or beef supplemented with synthetic CLA (c9 t11 CLA), for 28 days on molecular biomarkers of the metabolic syndrome, and adipose, hepatic, and skeletal muscle proteome in male ob/ob mice. Despite equal weight gain, CLA-fed mice had lower plasma glucose, insulin, non-esterified fatty acid, triacylglycerol and interleukin-6, and higher adiponectin concentrations than controls. c9 t11 CLA induced differential regulation of redox status across all tissues, and decreased hepatic and muscle endoplasmic reticulum stress. CLA also modulated mechanistic links between the actin cytoskeleton, insulin signalling, glucose transport and inflammation in the adipose tissue. In the liver and muscle, c9 t11 CLA improved metabolic flexibility through co-ordination between carbohydrate and energy metabolism. c9 t11 CLA may ameliorate systemic insulin sensitivity in obesity-induced diabetes by altering cellular stress and redox status, and modulating nutrient handling in key insulin-sensitive tissues through complex biochemical interplay among representative proteomic signatures.

Ancillary