Unraveling the resistance of microbial biofilms: Has proteomics been helpful?
Article first published online: 23 JAN 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Reviews 2012
Volume 12, Issue 4-5, pages 651–665, February 2012
How to Cite
Seneviratne, C. J., Wang, Y., Jin, L., Wong, S. S. W., Herath, T. D. K. and Samaranayake, L. P. (2012), Unraveling the resistance of microbial biofilms: Has proteomics been helpful?. Proteomics, 12: 651–665. doi: 10.1002/pmic.201100356
- Issue published online: 1 APR 2012
- Article first published online: 23 JAN 2012
- Accepted manuscript online: 13 JAN 2012 05:34AM EST
- Manuscript Accepted: 11 OCT 2011
- Manuscript Revised: 7 OCT 2011
- Manuscript Received: 30 JUN 2011
Biofilms are surface-attached, matrix-encased, structured microbial communities which display phenotypic features that are dramatically different from those of their free-floating, or planktonic, counterparts. Biofilms seem to be the preferred mode of growth of microorganisms in nature, and at least 65% of all human infections are associated with biofilms. The most notable and clinically relevant property of biofilms is their greater resistance to antimicrobials compared with their planktonic counterparts. Although both bacterial and fungal biofilms display this phenotypic feature, the exact mechanisms underlying their increased drug resistance are yet to be determined. Advances in proteomics techniques during the past decade have facilitated in-depth analysis of the possible mechanisms underpinning increased drug resistance in biofilms. These studies have demonstrated the ability of proteomics techniques to unravel new targets for combating microbial biofilms. In this review, we discuss the putative drug resistance mechanisms of microbial biofilms that have been uncovered by proteomics and critically evaluate the possible contribution of the new knowledge to future development in the field. We also summarize strategic uses of novel proteomics technologies in studies related to drug resistance mechanisms of microbial biofilms.