Separation of kallikrein 6 glycoprotein subpopulations in biological fluids by anion-exchange chromatography coupled to ELISA and identification by mass spectrometry
Version of Record online: 26 APR 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 6, pages 799–809, March 2012
How to Cite
Kuzmanov, U., Smith, C. R., Batruch, I., Soosaipillai, A., Diamandis, A. and Diamandis, E. P. (2012), Separation of kallikrein 6 glycoprotein subpopulations in biological fluids by anion-exchange chromatography coupled to ELISA and identification by mass spectrometry. Proteomics, 12: 799–809. doi: 10.1002/pmic.201100371
- Issue online: 26 APR 2012
- Version of Record online: 26 APR 2012
- Manuscript Accepted: 2 DEC 2011
- Manuscript Revised: 24 NOV 2011
- Manuscript Received: 8 JUL 2011
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|pmic7003-sup-0001-figures1.ppt||506K||Figure S1. Sample glycopeptide fragmentation. MS2 scans of the fragmentation of the triply charged glycopeptide at m/z = 1152.48. The fragmentation was performed in HCD mode with 17% (A), 24% (B), and 30% (C) normalized collision energy. The glycopeptide was also fragmented in CID mode with 35% normalized collision energy (D), as performed in the PIM methodology. The predicted charge states and fragmentation of selected m/z peaks are annotated.|
|pmic7003-sup-0001-figures1.ppt||506K||Figure S2. Composite MS1 spectra of rKLK6 anion-exchange chromatographic peaks. Combined MS1 spectra over the DCSANTTSCHILGWGK glycopeptide retention time period for recombinant KLK6 from different anion-exchange peaks. Spectra for peaks A-D are shown in Figure S2(A) to S2(D), respectively. The displayed structures reflect the predicted KLK6-attached glycans by composition, not linkage|
|pmic7003-sup-0001-figures1.ppt||506K||Table S1. Results of anion-exchange separation of KLK6 subpopulations in biological fluids and clinical information of patients. The data is represented as area of each chromatographic peak, expressed as a percentage (%) of total area under the curve.|
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