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pmic7003-sup-0001-figures1.ppt506KFigure S1. Sample glycopeptide fragmentation. MS2 scans of the fragmentation of the triply charged glycopeptide at m/z = 1152.48. The fragmentation was performed in HCD mode with 17% (A), 24% (B), and 30% (C) normalized collision energy. The glycopeptide was also fragmented in CID mode with 35% normalized collision energy (D), as performed in the PIM methodology. The predicted charge states and fragmentation of selected m/z peaks are annotated.
pmic7003-sup-0001-figures1.ppt506KFigure S2. Composite MS1 spectra of rKLK6 anion-exchange chromatographic peaks. Combined MS1 spectra over the DCSANTTSCHILGWGK glycopeptide retention time period for recombinant KLK6 from different anion-exchange peaks. Spectra for peaks A-D are shown in Figure S2(A) to S2(D), respectively. The displayed structures reflect the predicted KLK6-attached glycans by composition, not linkage
pmic7003-sup-0001-figures1.ppt506KTable S1. Results of anion-exchange separation of KLK6 subpopulations in biological fluids and clinical information of patients. The data is represented as area of each chromatographic peak, expressed as a percentage (%) of total area under the curve.

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