Colour Online: See the article online to view Figs. 1, 3, and 5 in colour.
Proteomic methods reveal cyclophilin a function as a host restriction factor against rotavirus infection
Version of Record online: 19 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 7, pages 1121–1132, April 2013
How to Cite
He, H., Mou, Z., Li, W., Fei, L., Tang, Y., Zhang, J., Yan, P., Chen, Z., Yang, X., Shen, Z., Li, J. and Wu, Y. (2013), Proteomic methods reveal cyclophilin a function as a host restriction factor against rotavirus infection. Proteomics, 13: 1121–1132. doi: 10.1002/pmic.201100579
- Issue online: 5 APR 2013
- Version of Record online: 19 FEB 2013
- Accepted manuscript online: 9 JAN 2013 09:55AM EST
- Manuscript Accepted: 17 DEC 2012
- Manuscript Revised: 28 AUG 2012
- Manuscript Received: 4 NOV 2011
- National Key Technology R&D Program of China. Grant Number: 2006BAI03B12
- National Natural Science Foundation of China. Grant Numbers: 30571708, 30490241, 81102233
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|pmic7341-sup-0001-FigureS1.doc||1265K||Figure S1. Differential expression profiles related to the upregulated protein spots. Each window corresponds to the gel region where the spot is located. In every two comparative windows, the left one is from mock infection and the right one is from HRV infection. The upregulated spots are circled and the numbers in the right correlate to those given in Fig. 1 and table 1.|
|pmic7341-sup-0002-FigureS2.doc||735K||Figure S2. MS spectra of the 16 protein spots successfully identified by MALDI-TOF-MS. The spot numbers correlate with those listed in Table 1 and Fig. 2. The matched peaks are labeled and their peptide assignments are listed.|
|pmic7341-sup-0003-FigureS3.doc||113K||Figure S3. MA104 cells infected by HRV (Wa strain) (moi = 3) and mock infected with DMEM were harvested at 12 h p.i. Then the protein in cytosol and nucleus were harvested using Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, China) respectively. These proteins were subjected to western blot analysis with the use of rabbit pAbs against CYPA (Santa Cruz Technology, USA). GAPDH and Lamin B were selected as control for the detection of CYPA in cytosol and nucleus respectively.|
|pmic7341-sup-0004-FigureS4.doc||35K||Figure S4. (A) Caco-2 cells infected by human rotavirus Wa strain were harvested at different time points (0 h, 12 h and 24 h p.i.) and subjected to western blot analysis with the use of rabbit pAbs against CYPA (Santa Cruz Technology, USA). GAPDH was detected for control. (B) MA104 cells infected by rotavirus Wa strain, SA11 strain and mock infected by DMEM were harvested at 12 h p.i. and subjected to western blot analysis with the use of rabbit pAbs against CYPA (Santa Cruz Technology, USA). β-actin was detected for control.|
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