A two-dimensional proteome map of the aflatoxigenic fungus Aspergillus flavus


Correspondence: Dr. Tibor Pechan, Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA

E-mail: pechan@ra.msstate.edu

Fax: +1-662-325-9264

Additional corresponding author: Dr. Ashli E. Brown,

E-mail: abrown@bch.msstate.edu


The filamentous fungus Aspergillus flavus is an opportunistic soil-borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel-based profiling analysis of A. flavus proteome and establishes a 2D proteome map. Using 2DE and MALDI-TOF-MS/MS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified.