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Figure S1. Detection of lysine-acetylated B. subtilis proteins by western blot analysis using 4 custom-made anti-acetyllysine antibodies and 1 commercial antibody. A, SDS-PAGE with Coomassie brilliant blue staining. B, western blot analysis with commercial antibody(Cell Signaling Co.): C, D, western blot analysis with independently generated anti-acetyllysine antibodies against the sequence 1 peptide; E, western blot analysis with anti-acetyllysine antibody generated using the sequence 2 peptide; F, western blot analysis with custom anti-acetyllysine antibody generated using the sequence 3 peptide. Lane M indicates the molecular weight marker and lane S indicates each protein lysate sample. Lane 1 and 2 indicate Ac-BSA and BSA in each panel, respectively.

Figure S2. Sequence logo plots represent the relative abundance for ±6 amino acids from the (A) E. coli, (B) S. enterica, (C) Mitochondria, (D) Human lysine-acetylated sites. The acetylproteome data is from three post-translational modification (PTM) database such as CPLA (, dbPTM (, and SysPTM (

pmic7405-sup-0002-FigureS3.doc6748KFigure S3. Annotated spectra identifying all 332 lysine acetylation sites. The raw MS/MS spectra of all the acetylated peptides annotated with the MASCOT search engine.
pmic7405-sup-0003-FigureS3.doc7188KFigure S3
pmic7405-sup-0004-FigureS3.doc4747KFigure S3
pmic7405-sup-0005-FigureS3.doc3933KFigure S3
pmic7405-sup-0006-Tables.doc1628KTables S1-S6.
pmic7405-sup-0007-TableS7.xls84KTable S7.

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