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The acetylproteome of Gram-positive model bacterium Bacillus subtilis
Article first published online: 3 JUN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Focus on Analysis of Extracellular Vesicles
Volume 13, Issue 10-11, pages 1726–1736, May 2013
How to Cite
Kim, D., Yu, B. J., Kim, J. A., Lee, Y.-J., Choi, S.-G., Kang, S. and Pan, J.-G. (2013), The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Proteomics, 13: 1726–1736. doi: 10.1002/pmic.201200001
- Issue published online: 3 JUN 2013
- Article first published online: 3 JUN 2013
- Accepted manuscript online: 6 MAR 2013 08:40AM EST
- Manuscript Accepted: 18 FEB 2013
- Manuscript Revised: 8 FEB 2013
- Manuscript Received: 2 JAN 2012
- Ministry of Science and Technology of Korea
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Figure S1. Detection of lysine-acetylated B. subtilis proteins by western blot analysis using 4 custom-made anti-acetyllysine antibodies and 1 commercial antibody. A, SDS-PAGE with Coomassie brilliant blue staining. B, western blot analysis with commercial antibody(Cell Signaling Co.): C, D, western blot analysis with independently generated anti-acetyllysine antibodies against the sequence 1 peptide; E, western blot analysis with anti-acetyllysine antibody generated using the sequence 2 peptide; F, western blot analysis with custom anti-acetyllysine antibody generated using the sequence 3 peptide. Lane M indicates the molecular weight marker and lane S indicates each protein lysate sample. Lane 1 and 2 indicate Ac-BSA and BSA in each panel, respectively.
Figure S2. Sequence logo plots represent the relative abundance for ±6 amino acids from the (A) E. coli, (B) S. enterica, (C) Mitochondria, (D) Human lysine-acetylated sites. The acetylproteome data is from three post-translational modification (PTM) database such as CPLA (http://cpla.biocuckoo.org), dbPTM (http://dbPTM.mbc.nctu.edu.tw), and SysPTM (http://www.sysbio.ac.cn/SysPTM).
|pmic7405-sup-0002-FigureS3.doc||6748K||Figure S3. Annotated spectra identifying all 332 lysine acetylation sites. The raw MS/MS spectra of all the acetylated peptides annotated with the MASCOT search engine.|
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