These authors contributed equally to this work.
Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice
Article first published online: 6 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 14, pages 2340–2354, August 2012
How to Cite
Li, Y., Zhang, Z., Nie, Y., Zhang, L. and Wang, Z. (2012), Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice. Proteomics, 12: 2340–2354. doi: 10.1002/pmic.201200054
Colour Online: See the article online to view Fig. 1 in colour.
- Issue published online: 6 AUG 2012
- Article first published online: 6 AUG 2012
- Accepted manuscript online: 25 JUN 2012 01:33AM EST
- Manuscript Accepted: 10 MAY 2012
- Manuscript Revised: 4 MAY 2012
- Manuscript Received: 12 FEB 2012
- National Natural Science Foundation of China. Grant Numbers: 30971887, 30600399
- Guangdong Natural Science Foundation. Grant Numbers: 10151064201000008, 07006683
- Magnaporthe oryzae;
- Plant proteomics;
- Salicylic acid
To probe salicylic acid (SA)-induced sequential events at translational level and factors associated with SA response, we conducted virulence assays and proteomic profiling analysis on rice resistant and susceptible cultivars against Magnaporthe oryzae at various time points after SA treatment. The results showed that SA significantly enhanced rice resistance against M. oryzae. Proteomic analysis of SA-treated leaves unveiled 36 differentially expressed proteins implicated in various functions, including defense, antioxidative enzymes, and signal transduction. Majority of these proteins were induced except three antioxidative enzymes, which were negatively regulated by SA. Consistent with the above findings, SA increased the level of reactive oxygen species (ROS) with resistant cultivar C101LAC showing faster response to SA and producing higher level of ROS than susceptible cultivar CO39. Furthermore, we showed that nucleoside diphosphate kinase 1, which is implicated in regulation of ROS production, was strongly induced in C101LAC but not in CO39. Taken together, the findings suggest that resistant rice cultivar might possess a more sensitive SA signaling system or effective pathway than susceptible cultivar. In addition, our results indicate that SA also coordinates other cellular activities such as photosynthesis and metabolism to facilitate defense response and recovery, highlighting the complexity of SA-induced resistance mechanisms.