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Table S1. Identification results of BSA digest after analyzed by CZE-ESI-MS/MS in triplicates.

Figure S1. Extracted spectra of one peptide (LKEccDKPLLEK, +2, m/z 766.8929) identified from different injection amount of BSA digest after analyzed by CZE-ESI-MS/MS. Injection amount of BSA digest: 3500, 1700, 700, 350, 120, and 60 amole (from top to bottom).

Figure S2. Linearity between injection amounts of BSA digest and peptide intensity. Error bars are ± 1 SD of the mean.

Figure S3. Linearity between injection amounts of myoglobin digest and peptide intensity after analysis by CZE-ESI-MS/MS in triplicate.

Figure S4. MS/MS spectrum of the identified peptide (LKEccDKPLLEK, +2, m/z 766.8929) from 60 amole BSA digest after analysis by CZE-ESI-MS/MS.

Figure S5. Number of peptide and spectral count from BSA digest after analysis by CZE-ESI-MS/MS and nanoLC-ESI-MS/MS in triplicate runs with same injection amount (A) and same BSA digest concentration (B).

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