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CZE-ESI-MS/MS system for analysis of subnanogram amounts of tryptic digests of a cellular homogenate
Article first published online: 29 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Focus on Emerging Gel-Free Separation and Detection Methods
Volume 12, Issue 19-20, pages 3013–3019, October 2012
How to Cite
Sun, L., Zhu, G., Li, Y., Wojcik, R., Yang, P. and Dovichi, N. J. (2012), CZE-ESI-MS/MS system for analysis of subnanogram amounts of tryptic digests of a cellular homogenate. Proteomics, 12: 3013–3019. doi: 10.1002/pmic.201200100
- Issue published online: 19 OCT 2012
- Article first published online: 29 AUG 2012
- Accepted manuscript online: 7 AUG 2012 08:44AM EST
- Manuscript Accepted: 18 JUN 2012
- Manuscript Revised: 14 JUN 2012
- Manuscript Received: 6 MAR 2012
- National Institutes of Health. Grant Number: R01GM096767
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Table S1. Identification results of BSA digest after analyzed by CZE-ESI-MS/MS in triplicates.
Figure S1. Extracted spectra of one peptide (LKEccDKPLLEK, +2, m/z 766.8929) identified from different injection amount of BSA digest after analyzed by CZE-ESI-MS/MS. Injection amount of BSA digest: 3500, 1700, 700, 350, 120, and 60 amole (from top to bottom).
Figure S2. Linearity between injection amounts of BSA digest and peptide intensity. Error bars are ± 1 SD of the mean.
Figure S3. Linearity between injection amounts of myoglobin digest and peptide intensity after analysis by CZE-ESI-MS/MS in triplicate.
Figure S4. MS/MS spectrum of the identified peptide (LKEccDKPLLEK, +2, m/z 766.8929) from 60 amole BSA digest after analysis by CZE-ESI-MS/MS.
Figure S5. Number of peptide and spectral count from BSA digest after analysis by CZE-ESI-MS/MS and nanoLC-ESI-MS/MS in triplicate runs with same injection amount (A) and same BSA digest concentration (B).
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