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Figure S1. Low angle X-ray diffraction (LAXRD) patterns of the C8-modified graphene@msilica composite materials

Figure S2. Wide angle XRD patterns of the C8-modified graphene@mSiO2 composite materials

Figure S3. N2 adsorption-desorption isotherms (insert) and pore size distribution of the C8-modified graphene@mSiO2 nanocomposites

Figure S4. FT-IR spectra of (a) the nitric acid treated graphene sample and (b) the C8-modified graphene@msilica composite materials

Figure S5. Photographs of the aqueous dispersion of (a) pristine graphene sheets, (b) nitric acid treated graphene and (c) C8-modified graphene@mSiO2 composites.

Figure S5. Mass spectra of 5 nM of standard peptides (Angiotensin II: Molecular Weight = 1046.2) eluated by different concentration of ammonia water. a) without any treatment; b) eluated by 0.4M of ammonia water; c) eluated by 1M of ammonia water; d) eluated by 2M of ammonia water.

Figure S6. Mass spectra of 5 nM of standard peptides (Angiotensin II: Molecular Weight = 1046.2) enriched by different amounts of C8-modified graphene@mSiO2 composites. a) 5 μL, 10mg/mL, in water; b) 10 μL, 10mg/mL, in water; c) 15 μL, 10mg/mL, in water; d) 20 μL, 10 mg/mL, in water.

Figure S7. Mass spectra of standard peptides (Angiotensin II: Molecular Weight = 1046.2) a)10 nM, b) 1 nM, c) 0.1 nM enriched by d) C8-modified graphene@mSiO2 composites (10 μL, 10mg/mL, in water)

Figure S8. Mass spectra of 5 nM standard peptides (Angiotensin II: Molecular Weight = 1046.2) enriched by C8-modified graphene@mSiO2 composites (10 μL, 10mg/mL, in water). a) the result of first application of the material, b) after eluting step, the result of second application of the material, c) after eluting step, the result of third application of the material, d) after eluting step, the result of fourth application of the material.

Figure S9. Mass spectra of 1mg/ml standard peptides (Angiotensin II: Molecular Weight = 1046.2) were enriched by C8-modified graphene@mSiO2 composites (10 μL, 10mg/mL, in water). a) Before the enrichment (S/N is 8924.66 and the concentration is 1mg/ml, 200μL) b) the supernatant after the enrichment (S/N is 6810.81 and the calculated concentration is 0.763 mg/ml, 200μL) c) the eluent after the enrichment (S/N is 3612.80 and the calculated concentration is 0.405 mg/ml, 10μL) The max loading capacity and the elution rate are calculated to be 4.737mg/mg and 85.5%, respectively.

Table S1. All peptides of mouse brain enriched by C8-modified graphene@mSiO2 and identified by 1D nano-ESI-LC/MS/MS.

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