Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy

Authors

  • Sun Hee Heo,

    1. Genome Research Center for Birth defects and Genetic Diseases, Asan Institute for Life Sciences, Asan Medical Center Children's Hospital, College of Medicine, University of Ulsan, Seoul, Korea
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    • Sun Hee Heo and Jin-Ho Choi contributed equally to this work.

  • Jin-Ho Choi,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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    • Sun Hee Heo and Jin-Ho Choi contributed equally to this work.

  • Yoo-Mi Kim,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Chang-Woo Jung,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Jin Lee,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Hye Young Jin,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Gu-Hwan Kim,

    1. Medical Genetics Center, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Beom Hee Lee,

    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
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  • Choong Ho Shin,

    1. Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea
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  • Han-Wook Yoo

    Corresponding author
    1. Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
    2. Medical Genetics Center, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea
    • Genome Research Center for Birth defects and Genetic Diseases, Asan Institute for Life Sciences, Asan Medical Center Children's Hospital, College of Medicine, University of Ulsan, Seoul, Korea
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  • Colour Online: See the article online to view Fig. 1 in colour.

Correspondence: Dr. Han-Wook Yoo, Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, 388-1, Pungnap-Dong, Songpa-Gu, Seoul 138-736, Korea

E-mail: hwyoo@amc.seoul.kr

Fax: +82 2 473 3725

Abstract

This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. They were treated with rhGH at a dose of 0.31 ± 0.078 mg/kg/week for 3 months. Immunodepletion of six highly-abundant serum proteins followed by 2D DIGE analysis, and subsequent MALDI TOF MS, were employed to generate a panel of proteins differentially expressed after short-term rhGH therapy and verify the differences in serum levels of specific proteins by rhGH therapy. Fourteen spots were differentially expressed after rhGH treatment. Among them, apo E and apo L-1 expression were consistently enhanced, whereas serum amyloid A was reduced after rhGH therapy. The differential expressions of these proteins were subsequently verified by Western blot analysis using sera of the before and after rhGH treatment. This study suggests that rhGH therapy influences lipoprotein metabolism and enhances apo L-1 protein expression in ISS patients.

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