These authors contributed equally to this work.
Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma
Article first published online: 19 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 22, pages 3416–3425, November 2012
How to Cite
Liu, Z., Chen, C., Yang, H., Zhang, Y., Long, J., Long, X. and Fang, W. (2012), Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma. Proteomics, 12: 3416–3425. doi: 10.1002/pmic.201200146
Colour Online: See the article online to view Figs. 1, 3, 4 and 6 in colour.
- Issue published online: 26 NOV 2012
- Article first published online: 19 OCT 2012
- Accepted manuscript online: 19 SEP 2012 07:08AM EST
- Manuscript Accepted: 3 SEP 2012
- Manuscript Revised: 6 AUG 2012
- Manuscript Received: 6 APR 2012
- National Nature Science Fund of China. Grant Numbers: 81071632, 30870973, 81101781
- Medical Science Research Fund of Guangdong Province. Grant Number: B2011133
- Medical College of Guangzhou. Grant Number: 2010C04
- Pearl River Science and Technology from Guangzhou City. Grant Number: 2011J2200009
- Guangdong Province Breeding. Grant Number: LYM11102
- Cell cycle;
- Nasopharyngeal carcinoma;
We previously defined the recently revised NESG1 gene as a potential tumor suppressor in nasopharyngeal carcinoma (NPC). Here, we further used proteomics technology to globally examine NESG1-controlled proteins in NPC cells. Twenty-six proteins were found to be deregulated by NESG1 using proteomics analysis while enolase 1 (alpha) (ENO1), heat shock protein 90 kDa beta (Grp94), member 1 (HSP90B1), and cathepsin D (CTSD) proteins were differentially expressed by Western blot. Interestingly, a-enolase (ENO1), an overexpressed gene in NPC, was confirmed as a NESG1-regulated protein in NPC cells. Overexpressed ENO1 not only restored cell proliferation and cell-cycle progression, but also antagonized the regulation of NESG1 to cell-cycle regulators p21 and CCNA1 expression as well as induced the expression of C-Myc, pRB, and E2F1 in NESG1-ovexpressed NPC cells. Real-time PCR and immunohistochemistry analysis showed that NESG1 expression is negatively correlated with ENO1 expression in NPC tissues. Our observations suggest that ENO1 downregulation plays an important role in NESG1-induced growth inhibition of NPC cancer cells.