These authors contributed equally to this study.
Protein profiling of epidermal bladder cells from the halophyte Mesembryanthemum crystallinum
Article first published online: 22 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 18, pages 2862–2865, September 2012
How to Cite
Barkla, B. J., Vera-Estrella, R. and Pantoja, O. (2012), Protein profiling of epidermal bladder cells from the halophyte Mesembryanthemum crystallinum. Proteomics, 12: 2862–2865. doi: 10.1002/pmic.201200152
Colour Online: See the article online to view Figs. 1–3 in colour.
- Issue published online: 12 SEP 2012
- Article first published online: 22 AUG 2012
- Accepted manuscript online: 27 JUL 2012 07:11AM EST
- Manuscript Accepted: 18 JUL 2012
- Manuscript Revised: 27 JUN 2012
- Manuscript Received: 18 APR 2012
- UNAM-DGAPA-PAPIIT. Grant Number: 212410
- Crassulacean acid metabolism;
- Epidermal bladder cells;
- Plant proteomics;
- Salt tolerance
Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been proposed to have roles in plant defense and UV protection, but primarily in sodium sequestration and as water reservoirs. To gain further understanding into the roles of EBC, a cell-type-specific proteomics approach was taken in which precision single-cell sampling of cell sap from individual EBC was combined with shotgun peptide sequencing (LC-MS/MS). Identified proteins showed diverse biological functions and cellular locations, with a high representation of proteins involved in H+-transport, carbohydrate metabolism, and photosynthesis. The proteome of EBC provides insight into the roles of these cells in ion and water homeostasis and raises the possibility that they are photosynthetically active and functioning in Crassulacean acid metabolism.