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pmic7248-sup-0001-TableS1.doc112K

Table S1. Differentially expressed proteins identified by MALDI-TOF in barley leaves. The differentially expressed leaf protein spots were identified between a drought tolerant (Basrah, B) and a drought susceptible (Golden Promise, GP) barley variety using 2D-DIGE. The differentially expressed leaf protein spots were analysed by MALDI-TOF. The protein expression patterns (change in protein expression between GP and B before and after exposure to drought), were divided into categories as shown in Fig.5; fold expression, the change in expression level of the analysed protein when compared between two treatment groups; T-test, shows the treatment groups compared when analysing significance of differences in protein expression; p-value is the significance level of the T-test; annotation, the identity of the protein as determined by Mascot; Acc #, the NCBI accession number; species is that relating to the accession number; Blast, the identity of proteins closely related (>80% homology) to the protein identified by MALDI-TOF;% sequence cov/ No. of peptides, the degree of coverage and the number of matched peptides of the identified protein; E-value, the probability of a random match by Mascot.

Table S2. Differentially expressed proteins identified by MALDI-TOF in barley roots. The differentially expressed leaf protein spots were identified between a drought tolerant (Basrah, B) and a drought susceptible (Golden Promise, GP) barley variety using 2D-DIGE. The differentially expressed root protein spots were analysed by MALDI-TOF. The protein expression patterns (change in protein expression between GP and B before and after exposure to drought), were divided into categories as shown in Fig.5; fold expression, the change in expression level of the analysed protein when compared between two treatment groups; T-test, shows the treatment groups compared when analysing significance of differences in protein expression; p-value is the significance level of the T-test; annotation, the identity of the protein as determined by Mascot; Acc #, the NCBI accession number; species is that relating to the accession number; Blast, the identity of proteins closely related (>80% homology) to the protein identified by MALDI-TOF;% sequence cov/ No. of peptides, the degree of coverage and the number of matched peptides of the identified protein; E-value, the probability of a random match by Mascot.

pmic7248-sup-0002-FigureS1.pdf11930KFigure S1. 2D-DIGE electrophoretic pattern of leaf protein extracts. Panel A shows all three scans superimposed. Equal amounts of proteins from all treatments were labelled with Cy2 (blue, B), proteins extracted from drought-treated Golden Promise leaves were labelled with Cy3 (green, C) and proteins extracted from drought-treated Basrah leaves were labelled with Cy5 (red, D).
pmic7248-sup-0003-FigureS2.doc2900KFigure S2. 2D-DIGE electrophoretic pattern of root protein extracts. Panel A shows all three scans superimposed. Equal amounts of proteins from all treatments were labelled with Cy2 (blue, B), proteins extracted from drought-treated Golden Promise roots were labelled with Cy3 (green, C) and proteins extracted from drought-treated Basrah roots were labelled with Cy5 (red, D).

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