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Document S1. AMT tag Alignment and Mass Validation.

Supporting Information Table 1. Sequence and number of unique peptides for all the soybean proteins.

Table S2. Accurate mass and time (AMT) tag library.

Table S3. Abundance of peptides detected in root hairs and stripped roots.

Table S4. Abundance of proteins detected in root hairs and stripped roots.

Table S5. Annotation of proteins detected in root hairs and stripped roots and identified with at least 2 peptides.

Table S6. Proteins preferentially detected in root hairs (never detected in stripped roots).

Table S7. Proteins detected in root hairs and already described to play a role during the nitrogen fixing symbiosis process.

Figure S1. The relationship between the number of proteins and the number of unique peptides generated by in silico digestion of the soybean proteome using various proteolytic enzymes.

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