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CPhos: A program to calculate and visualize evolutionarily conserved functional phosphorylation sites

Authors

  • Boyang Zhao,

    1. National Heart, Lung, and Blood Institute (NHLBI), Epithelial Systems Biology Laboratory,, National Institutes of Health (NIH), Bethesda, MD, USA
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  • Trairak Pisitkun,

    1. National Heart, Lung, and Blood Institute (NHLBI), Epithelial Systems Biology Laboratory,, National Institutes of Health (NIH), Bethesda, MD, USA
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  • Jason D. Hoffert,

    1. National Heart, Lung, and Blood Institute (NHLBI), Epithelial Systems Biology Laboratory,, National Institutes of Health (NIH), Bethesda, MD, USA
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  • Mark A. Knepper,

    1. National Heart, Lung, and Blood Institute (NHLBI), Epithelial Systems Biology Laboratory,, National Institutes of Health (NIH), Bethesda, MD, USA
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  • Fahad Saeed

    Corresponding author
    • National Heart, Lung, and Blood Institute (NHLBI), Epithelial Systems Biology Laboratory,, National Institutes of Health (NIH), Bethesda, MD, USA
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  • Colour Online: See the article online to view Figs. 1–3 in colour.

Correspondence: Dr. Fahad Saeed, National Institutes of Health (NIH), Building 10, Room 6N312 MSC-1603, Bethesda, Maryland 20892–1603, USA

E-mail: fahad.saeed@nih.gov

Fax: +1 301-402-1443

Abstract

Profiling using high-throughput MS has discovered an overwhelming number of novel protein phosphorylation sites (“phosphosites”). However, the functional relevance of these sites is not always clear. In light of recent studies on the evolutionary mechanism of phosphorylation, we have developed CPhos, a Java program that can assess the conservation of phosphosites among species using an information theory-based approach. The degree of conservation established using CPhos can be used to assess the functional significance of phosphosites. CPhos has a user friendly graphical user interface and is available both as a web service and as a standalone Java application to assist phosphoproteomic researchers in analyzing and prioritizing lists of phosphosites for further experimental validation. CPhos can be accessed or downloaded at http://helixweb.nih.gov/CPhos/.

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