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Simultaneous extraction and rapid visualization of peptidomic and lipidomic body fluids fingerprints using mesoporous aluminosilicate and MALDI-TOF MS
Article first published online: 19 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 22, pages 3286–3294, November 2012
How to Cite
Preianò, M., Pasqua, L., Gallelli, L., Galasso, O., Gasparini, G., Savino, R. and Terracciano, R. (2012), Simultaneous extraction and rapid visualization of peptidomic and lipidomic body fluids fingerprints using mesoporous aluminosilicate and MALDI-TOF MS. Proteomics, 12: 3286–3294. doi: 10.1002/pmic.201200204
- Issue published online: 26 NOV 2012
- Article first published online: 19 OCT 2012
- Accepted manuscript online: 19 SEP 2012 07:08AM EST
- Manuscript Accepted: 5 SEP 2012
- Manuscript Revised: 1 AUG 2012
- Manuscript Received: 16 MAY 2012
- MALDI-TOF MS;
- Mesoporous materials;
Herein we report the use of mesoporous aluminosilicate (MPAS) for the simultaneous extraction of peptides and lipids from complex body fluids such as human plasma and synovial fluid. We show that MPAS particles, given their mesostructural features with nanometric pore size and high surface area, are an efficient device for simultaneous extraction of peptidome and lipidome from as little as a few microliters of body fluids. The peptides and the lipids, selected and enriched by MPAS particles and rapidly visualized by MALDI-TOF MS, could form part of a diagnostic profile of the “peptidome” and the “lipidome” of healthy versus diseased subjects in comparative studies. The ability of this approach to rapidly reveal the overall pattern of changes in both lipidome and peptidome signatures of complex biofluids could be of valuable interest for handling large numbers of samples required in -omics studies for the purpose of finding novel biomarkers.