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Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease
Article first published online: 5 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 21, pages 3193–3205, November 2012
How to Cite
Li, S.-W., Yang, T.-C., Wan, L., Lin, Y.-J., Tsai, F.-J., Lai, C.-C. and Lin, C.-W. (2012), Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease. Proteomics, 12: 3193–3205. doi: 10.1002/pmic.201200225
- Issue published online: 29 OCT 2012
- Article first published online: 5 OCT 2012
- Accepted manuscript online: 31 AUG 2012 05:01AM EST
- Manuscript Accepted: 9 AUG 2012
- Manuscript Revised: 20 JUL 2012
- Manuscript Received: 5 JUN 2012
- National Science Council (Taiwan) and China Medical University. Grant Numbers: NSC101-2320-B-039-036-MY3, CMU98-P-03-M, CMU99-NSC-08
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Table S1. Primers used in this study
Fig. S1. Tandem mass spectrometry spectra of up-regulated proteins induced by PLpro. (A) Nanoelectrospray mass spectrum of doubly charged ion m/z 547.2859 for spot ID 7 is shown, amino acid sequence FADLSEAANR determined from mass differences in y- and b-fragment ions series and matched residues 295–304 of vimentin. (B) Nanoelectrospray mass spectrum of the doubly charged ion m/z 639.4361 for spot ID 17 is shown, amino acid sequence LALDIEIATYR determined from mass differences in y- and b-fragment ions series and matched residues 357–367 of glial fibrillary acidic protein. (C) Nanoelectrospray mass spectrum of doubly charged ion m/z 582.3241 for spot ID 1 is shown, amino acid sequence LFDQAFGLPR determined from mass differences in y- and b-fragment ions series and matched residues 28–37 of heat shock protein 27. *Only y- and b-fragment ions are labeled in the spectrum.
Fig. S2. Analysis of PDIA3 and UBE2K mRNA levels in vector control and PLpro-expressing cells. Total RNA was extracted from vector control and PLpro-expressing cells. The mRNA expression of PDIA3 and UBE2K was measured by quantitative real-time PCR. The relative fold levels of PDIA3 and UBE2K mRNA levels are presented as the ratio with GAPDH mRNA. Each bar on the graph is the mean of 3 independent experiments and the error bars represent the standard error of the mean.
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