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Table S1. Primers used in this study

Fig. S1. Tandem mass spectrometry spectra of up-regulated proteins induced by PLpro. (A) Nanoelectrospray mass spectrum of doubly charged ion m/z 547.2859 for spot ID 7 is shown, amino acid sequence FADLSEAANR determined from mass differences in y- and b-fragment ions series and matched residues 295–304 of vimentin. (B) Nanoelectrospray mass spectrum of the doubly charged ion m/z 639.4361 for spot ID 17 is shown, amino acid sequence LALDIEIATYR determined from mass differences in y- and b-fragment ions series and matched residues 357–367 of glial fibrillary acidic protein. (C) Nanoelectrospray mass spectrum of doubly charged ion m/z 582.3241 for spot ID 1 is shown, amino acid sequence LFDQAFGLPR determined from mass differences in y- and b-fragment ions series and matched residues 28–37 of heat shock protein 27. *Only y- and b-fragment ions are labeled in the spectrum.

Fig. S2. Analysis of PDIA3 and UBE2K mRNA levels in vector control and PLpro-expressing cells. Total RNA was extracted from vector control and PLpro-expressing cells. The mRNA expression of PDIA3 and UBE2K was measured by quantitative real-time PCR. The relative fold levels of PDIA3 and UBE2K mRNA levels are presented as the ratio with GAPDH mRNA. Each bar on the graph is the mean of 3 independent experiments and the error bars represent the standard error of the mean.

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